Pablo Blinder, PhD

 
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Address:
Dept. of Neurobiology
Room 711, Sherman building
Tel Aviv University
Tel-Aviv 69978
Israel

Homepage: pblab.tau.ac.il

MAIN AREAS OF RESEARCH

Proper brain function is mediated by the local control of blood flow (LCBF), requiring an intricate coupling between the neuronal and the vascular networks. I seek to understand the nature of this interface applying a combined functional, structural and molecular approach.

Part of my doctorate thesis focused on the self-organization of cultured neuronal networks and revealed a novel mechanism that links network structure with synaptogenesis. In the course of my PhD, I mastered techniques related to primary cell culture, plated-cell electrophysiology, immuno-histochemistry, imaging synaptic vesicle dynamics and calcium imaging of cultured neurons.

I have devoted my post-doctoral research to the study of morphological and functional aspects of the interface between neuronal and vascular network in the murine cortex. I have elucidated key structural features of the murine pial vasculature behind its ability to provide robust blood supply. This multidisciplinary project combined in vivo imaging of blood flow dynamics, graph-theory based anatomical reconstruction of entire pial arterial networks and targeted photo-thrombosis insults designed to challenge perfusion in the pial vasculature.

In my main post-doctoral research project, I have performed block-face imaging to acquire several cubic millimeters of contiguous histological data to reconstruct large portions of the murine somatosensory cortex, including all neurons and the surrounding vasculature. This unique datasets allows to study the link between cortical columns and the organization of the vasculature around them. This project combined the use of different techniques such as two-photon based high-throughput histology combined with laser-assisted ablation, intrinsic signal imaging, rheological modeling in large 3D realistic networks and spectral analysis of network structure.

Research Experience:

Start Oct 2012: Junior PI, Dept. of Neurobiology, Tel Aviv University, Israel

Aug 2006 – present: Post-doctoral Fellow

Neurophysics Laboratory with Prof. David Kleinfeld. Physics Dept., Univ. of California, San Diego Research in the area of cortical neurovascular coupling involving high-throughput all optical histology for large scale reconstruction of the mouse vasculature and cellular architecture in the somatosensory cortex and in vivo cortical imaging for blood flow and cellular dynamics.

2005 – 2006 Associated researcher, The Biophysical Interdisciplinary Schottenstein Center for the Research and Technology of the Cellome.

 

Most important 5-10 publications
Cohen E, Paulsson JF, Blinder P, Burstyn-Cohen T, Du D, Estepa G, Adame A, Pham HM, Holzenberger M, Kelly JW, Masliah E and Dillin A (2009). Reduced IGF-1 Signaling Delays Age-associated Proteotoxicity in Mice. Cell 139(6): 1157-1169

Tsai PS, Kaufhold JK, Blinder P, Friedman B, Drew PJ, Karten HJ, Lyden PD and Kleinfeld D (2009). Correlations of neuronal and microvascular densities in mrine cortex revealed by direct counting and colocalization of nuclei and vessels. J Neurosci. 29(46):14553- 70.

Blinder P*, Shih AY*, Rafie CA and Kleinfeld D (2010). Topological basis for the robust distribution of blood to rodent neocortex. PNAS 107(28): 12670-12675. *Equal contribution

Drew PJ, Shih AY, Tsai PS, Knutsen P, Davalos D, Blinder P, Akassoglou K and Kleinfeld D (2010). Large-scale chronic imaging and optical manipulation of cortical cells through a polished and reinforced thinned-skull. Nature Methods. 7: 981-984.

Andy Y Shih, Pablo Blinder, Philbert S Tsai, Beth Friedman, Geoffrey Stanley, and Patrick D Lyden. The smallest stroke : occlusion of one penetrating vessel leads to infarction and a cognitive deficit. Nature neuroscience, 16(1):55:63, Jan 2013

Pablo Blinder*, Philbert S Tsai*, John P Kaufhold, Per Magne-Knutsen, Hary Suhl and David Kleinfeld. The cortical angiome: A 3-D interconnected vascular network with noncolumnar patterns of blood flow. Nature neuroscience, 16, 889:897. *equal collaboration.