Mario Mairhofer, PhD

 
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Address:
Medical University of Vienna
Department of Gynecology & Obstetrics,
Research Laboratory 5Q,
Waehringer Guertel 18-20,
A-1090 Vienna

 
MAIN AREAS OF RESEARCH

I am a biochemist / molecular biologist with 10 years of experience in blood cell biochemistry (erythrocytes and platelets), in-vitro cultivation of primary erythroid progenitor cells and hematopoietic stem cells. During my diploma thesis and PhD thesis in the laboratory of Rainer Prohaska, my research was focused on the biochemical and cell biological investigation of stomatin family proteins. These enigmatic membrane micro-domain associated proteins are involved in cholesterol homeostasis and vesicular trafficking. After my PhD thesis, I joined the Laboratory of Andrea Kolbus at the Department of Obstetrics and Gynecology in 2007 to work on primary hematopoietic / erythroid progenitor cells from cord blood in the EC-funded project CRYSTAL (Cryopreservation of Stem Cells for Therapeutical Application). For the EC-funded project HYPERLAB, we are currently trying to optimize current protocols for the ex vivo expansion of hematopoietic stem/progenitor cells. We have long-standing experience and local collaborations in the cultivation and differentiation of erythroid progenitor cells in-vitro. In addition, we have established methods to transiently or stably interfere with gene expression in primary erythroid cells (by electroporation or lentiviral transduction). Our well-equipped laboratory is located at the General Hospital of Vienna, which gives us access to all core facilities (Cell Sorting, Tissue Imaging, Gene Expression, Proteomics) of the Medical University of Vienna.

Research Epxerience

2007 – Postdoctoral Fellow Medical University of Vienna, Dept. Of Obstetrics and Gynecology,Research Interest: Mechanisms guiding terminal erythroid differentiation in primary human cells, Supervisor: Priv. Doz. Dr. Andrea Kolbus

2002-2007 PhD thesis in Molecular Biology (PhD awarded April 2008) Max F. Perutz Laboratories Vienna, Thesis title: “Cell biological characterization of SLP-1 and stomatin.” Supervisor: Ao. Univ. Prof. Dr. Rainer Prohaska

 

Most important 5-10 publications

Mairhofer M, Schulz JC, Parth M, Beer U, Zimmermann H, Kolbus A. Evaluation of a xeno-free protocol for long-term cryopreservation of cord blood cells. Cell Transplant. 2013;22(6):1087-99.

Zeisberger SM§, Schulz JC§, Mairhofer M§, Ponsaerts P, Wouters G, Doerr D, Katsen-Globa A, Ehrbar M, Hescheler J, Hoerstrup SP, Zisch AH, Kolbus A, Zimmermann H. Biological and physicochemical characterization of a serum- andxeno-free chemically defined cryopreservation procedure for adult human progenitor cells. Cell Transplant. 2011;20(8):1241-57.

§...equal contribution, shared 1st authorship

Mairhofer M, Steiner M, Salzer U, Prohaska R. Stomatin-like protein-1 interacts with stomatin and is targeted to late endosomes. J Biol Chem. 2009 Oct 16;284(42):29218-29.

Mairhofer M, Steiner M, Mosgoeller W, Prohaska R, Salzer U. Stomatin is a major lipid-raft component of platelet alpha granules. Blood. 2002 Aug 1;100(3):897-904.

Umlauf E, Mairhofer M, Prohaska R. Characterization of the stomatin domain involved in homo-oligomerization and lipid raft association. J Biol Chem. 2006 Aug 18;281(33):23349-56.

Sanidas I, Kotoula V, Ritou E, Daans J, Lenz C, Mairhofer M, Daniilidou M, Kolbus A, Kruft V, Ponsaerts P, Nikolakaki E. The ratio of SRPK1/SRPK1a regulates erythroid differentiation in K562 leukaemic cells. Biochim Biophys Acta. 2010 Dec;1803(12):1319-31.

 

Pietrowski D, Gong Y, Mairhofer M, Gessele R, Sator M. Effects of Progesterone and its Metabolites on Human Granulosa Cells. Horm Metab Res. 2013 Oct 17. [Epub ahead of print] PubMed PMID: 24136781.

Fock V, Mairhofer M, Otti GR, Hiden U, Spittler A, Zeisler H, Fiala C, Knöfler M, Pollheimer J. Macrophage-derived IL-33 is a critical factor for placental growth. J Immunol. 2013 Oct 1;191(7):3734-43. doi: 10.4049/jimmunol.1300490. Epub 2013 Aug 30. PubMed PMID: 23997215.